SARS-CoV-2 infections pose a global threat to human health and an unprecedented research challenge. Among the most urgent tasks is obtaining a detailed understanding of the molecular interactions that facilitate viral replication or contribute to host defense mechanisms in infected cells.
While SARS-CoV-2 co-opts cellular factors for viral translation and genome replication, a comprehensive map of the host cell proteome in direct contact with viral RNA has not been elucidated.
Now, HIRI scientist Mathias Munschauer and colleagues established an unbiased and quantitative picture of the human proteome that directly binds the SARS-CoV-2 RNA in infected human cells.
The SARS-CoV-2 RNA interactome provides an unprecedented molecular perspective on SARS-CoV-2 infections and enables the systematic dissection of host dependency factors and host defense strategies, a crucial prerequisite for designing novel therapeutic strategies.
Browse the interactive RNA-proteome network in the online resource
A direct RNA-protein interaction atlas of the SARS-CoV-2 RNA in infected human cells
Nora Schmidt, Caleb A Lareau, Hasmik Keshishian, Randy Melanson, Matthias Zimmer, Luisa Kirschner, Jens Ade, Simone Werner, Neva Caliskan, Eric S Lander, Joerg Vogel, Steven A Carr, Jochen Bodem, Mathias Munschauer DOI: 10.1101/2020.07.15.204404