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Publications

Publication Highlights

Have a look at our latest discoveries.

2020

Methods for characterizing, applying, and teaching CRISPR-Cas systems

Beisel C (2020)

Methods (San Diego, Calif.)DOI: 10.1016/j.ymeth.2020.01.004

CRISPR-Cas Systems and the Paradox of Self-Targeting Spacers

Wimmer F, Beisel C (2020)

Front. Microbiol., 10: 1365DOI: 10.3389/fmicb.2019.03078

2019

Barriers to genome editing with CRISPR in bacteria

Vento J, Crook N, Beisel C (2019)

J Ind Microbiol Biotechnol, 46 (9-10): 1327-1341DOI: 10.1007/s10295-019-02195-1

Rapid transcriptional responses to serum exposure are associated with sensitivity and resistance to antibody-mediated complement killing in invasive Salmonella Typhimurium ST313

Ondari E, Klemm E, Msefula C, El Ghany M, Heath J, Pickard D, Barquist L, Dougan G, Kingsley R, MacLennan C (2019)

Wellcome Open Res, 4: 74DOI: 10.12688/wellcomeopenres.15059.1

The CRISPR/Cas system in Neisseria meningitidis affects bacterial adhesion to human nasopharyngeal epithelial cells

Heidrich N, Hagmann A, Bauriedl S, Vogel J, Schoen C (2019)

RNA biology, 16 (4): 390-396DOI: 10.1080/15476286.2018.1486660

Modular one-pot assembly of CRISPR arrays enables library generation and reveals factors influencing crRNA biogenesis

Liao C, Ttofali F, Slotkowski R, Denny S, Cecil T, Leenay R, Keung A, Beisel C (2019)

Nature Communications, 10 (1): 2948DOI: 10.1038/s41467-019-10747-3

Thermodynamic control of -1 programmed ribosomal frameshifting

Bock L, Caliskan N, Korniy N, Peske F, Rodnina M, Grubmüller H (2019)

Nature Communications, 10 (1): 4598DOI: 10.1038/s41467-019-12648-x

Targeted transcriptional modulation with type I CRISPR-Cas systems in human cells

Pickar-Oliver A, Black J, Lewis M, Mutchnick K, Klann T, Gilcrest K, Sitton M, Nelson C, Barrera A, Bartelt L, …, Barrangou R, Gersbach C (2019)

Nat BiotechnolDOI: 10.1038/s41587-019-0235-7

Transcriptional noise and exaptation as sources for bacterial sRNAs

Jose B, Gardner P, Barquist L (2019)

Biochemical Society transactions, 47 (2): 527-539DOI: 10.1042/BST20180171

Characterization of the all-E. coli transcription-translation system myTXTL by mass spectrometry

Garenne D, Beisel C, Noireaux V (2019)

Rapid Communications In Mass Spectrometry, 33 (11): 1036-1048DOI: 10.1002/rcm.8438

The conserved 3' UTR-derived small RNA NarS mediates mRNA crossregulation during nitrate respiration

Wang C, Chao Y, Matera G, Gao Q, Vogel J (2019)

Nucleic Acids ResDOI: 10.1093/nar/gkz1168

scSLAM-seq reveals core features of transcription dynamics in single cells

Erhard F, Baptista M, Krammer T, Hennig T, Lange M, Arampatzi P, Jürges C, Theis F, Saliba A, Dölken L (2019)

Nature, 571 (7765): 419-423DOI: 10.1038/s41586-019-1369-y

The Major RNA-Binding Protein ProQ Impacts Virulence Gene Expression in Salmonella enterica Serovar Typhimurium

Westermann A, Venturini E, Sellin M, Förstner K, Hardt W, Vogel J (2019)

MBio, 10 (1): 02504-18DOI: 10.1128/mBio.02504-18

Induced Pluripotent Stem Cell-Derived Brain Endothelial Cells as a Cellular Model to Study Neisseria meningitidis Infection

Martins Gomes S, Westermann A, Sauerwein T, Hertlein T, Förstner K, Ohlsen K, Metzger M, Shusta E, Kim B, Appelt-Menzel A, Schubert-Unkmeir A (2019)

Front Microbiol, 10: 1181DOI: 10.3389/fmicb.2019.01181

Distinct timescales of RNA regulators enable the construction of a genetic pulse generator

Westbrook A, Tang X, Marshall R, Maxwell C, Chappell J, Agrawal D, Dunlop M, Noireaux V, Beisel C, Lucks J, Franco E (2019)

Biotechnol Bioeng, 116 (5): 1139-1151DOI: 10.1002/bit.26918

Functional expansion of a TCA cycle operon mRNA by a 3' end-derived small RNA

Miyakoshi M, Matera G, Maki K, Sone Y, Vogel J (2019)

Nucleic Acids Res, 47 (4): 2075-2088DOI: 10.1093/nar/gky1243

An enhanced assay to characterize anti-CRISPR proteins using a cell-free transcription-translation system

Wandera K, Collins S, Wimmer F, Marshall R, Noireaux V, Beisel C (2019)

Methods (San Diego, Calif.) (19): 1046-2023DOI: 10.1016/j.ymeth.2019.05.014

The evolution of RNA structural probing methods: From gels to next-generation sequencing

Mailler E, Paillart J, Marquet R, Smyth R, Vivet-Boudou V (2019)

Wiley interdisciplinary reviews. RNA, 10 (2): 1518DOI: 10.1002/wrna.1518

CRATES: A one-step assembly method for Class 2 CRISPR arrays

Liao C, Slotkowski R, Beisel C (2019)

Methods Enzymol, 629: 493-511DOI: 10.1016/bs.mie.2019.04.011

The Acidaminococcus sp. Cas12a nuclease recognizes GTTV and GCTV as non-canonical PAMs

Jacobsen T, Liao C, Beisel C (2019)

FEMS microbiology letters, 366 (8): 085DOI: 10.1093/femsle/fnz085

An RNA Surprise in Bacterial Effector Mechanisms

Gerovac M, Vogel J (2019)

Cell Host Microbe, 26 (6): 709-711DOI: 10.1016/j.chom.2019.11.005

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