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Dr Chunyu Liao

About

Chunyu obtained her PhD degree in Microbiology from Iowa State University in December 2015. During her PhD study with Dr Laura Jarboe, she worked on exploring genetic factors driving attachment of bacteria to environmental particles. As a PostDoc in the Beisel lab, her current research focused on investigating mechanisms of crRNA biogenesis and developing tools for CRISPR-based high-throughput screens.


2020

Characterization of Cas12a nucleases reveals diverse PAM profiles between closely-related orthologs

Jacobsen T, Ttofali F, Liao C, Manchalu S, Gray BN, Beisel CL (2020)

Nucleic Acids Research, 48 (10): 5624-5638DOI: 10.1093/nar/gkaa272

2019

CRATES: A one-step assembly method for Class 2 CRISPR arrays

Liao C, Slotkowski RA, Beisel CL (2019)

Methods In Enzymology, 629: 493-511DOI: 10.1016/bs.mie.2019.04.011

Modular one-pot assembly of CRISPR arrays enables library generation and reveals factors influencing crRNA biogenesis

Liao C, Ttofali F, Slotkowski RA, Denny SR, Cecil TD, Leenay RT, Keung AJ, Beisel CL (2019)

Nature Communications, 10 (1): 2948DOI: 10.1038/s41467-019-10747-3

The Acidaminococcus sp. Cas12a nuclease recognizes GTTV and GCTV as non-canonical PAMs

Jacobsen T, Liao C, Beisel CL (2019)

FEMS Microbiology Letters, 366 (8): 085DOI: 10.1093/femsle/fnz085

2018

The Francisella novicida Cas12a is sensitive to the structure downstream of the terminal repeat in CRISPR arrays

Liao C, Slotkowski RA, Achmedov T, Beisel CL (2018)

RNA Biology, 16 (4): 404-412DOI: 10.1080/15476286.2018.1526537

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