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This guide outlines the process of using our single-cell facility, from initial consultation to final data delivery. We offer our users with varying levels of single-cell experience tailored solutions to meet diverse project needs.
1. Initial Consultation & Project Definition
We understand that every research project is unique. Therefore, we offer two entry points:
• Direct Analysis Download: Users with established single-cell analysis pipelines and familiarity with our facility can directly download analysis order forms.
• Application Specialist Consultation: For users seeking guidance or requiring customized solutions, we strongly recommend contacting our application specialists. This consultation will help determine the optimal approach for your project, including experimental design, library preparation methods, and bioinformatic analysis strategies.
2. Quotation & Agreement
Following the consultation or submission of the analysis order form, you will receive:
• Quotation: A detailed breakdown of the costs associated with your project.
• Agreement Sheet: A formal document outlining the terms and conditions of our services.
Please review both documents carefully. Signing and returning the agreement sheet confirms your project and authorizes us to proceed.
3. Sample Submission Scheduling
After receiving the signed agreement, we will contact you to schedule a date and time for sample submission. This step is crucial for ensuring a smooth and efficient workflow, minimizing sample degradation, and optimizing processing times. We will work with you to find a mutually convenient time.
4. Sample Processing
Upon arrival, your samples will undergo the following steps:
• Quality Control (QC): If sufficient material is available, we will perform a quality check to assess sample integrity and viability. This ensures that only high-quality samples proceed to the next stage.
• Library Preparation: Based on the agreed-upon method, we will prepare sequencing libraries from your single cells. This involves isolating RNA, reverse transcription, cDNA amplification, and adapter ligation.
• Library QC: Following library preparation, we perform another round of QC to assess library quality and concentration, ensuring optimal sequencing performance.
5. Sequencing & Bioinformatic Analysis
• Sequencing: The prepared libraries will be sequenced using our state-of-the-art sequencing platform.
• Preliminary Bioinformatic Analysis: After sequencing, the raw data will undergo preliminary bioinformatic analysis, including demultiplexing, read alignment, and gene expression quantification.
6. Data Delivery
The processed data, along with a summary report of the preliminary bioinformatic analysis and QC metrics, will be securely transferred to you via the institutional server.
